In this interactive activity adapted from the University of Nebraska's Plant and Soil Science eLibrary, learn how DNA is extracted from leaf tissue for molecular techniques, such as PCR. As the animated sequence shows, DNA extraction involves collecting cells, physically breaking them open to release DNA from the nuclei, separating the DNA from other cellular components, and precipitating DNA out of solution for final extraction. The process utilizes basic laboratory tools, such as microfuge tubes, pipettes, and a centrifuge. Other items used in purifying DNA include buffer solution, a solvent such as chloroform, and ethanol.
This media asset was adapted from Plant and Soil Sciences eLibrary DNA Extraction.